PENGUJIAN AKTIVITAS ANTIBAKTERI RERUMPUTAN PAKAN TERNAK TERHADAP Shigella dysentriae
| dc.contributor.advisor | Tidak ada Data Dosen | |
| dc.contributor.advisor | Tidak ada Data Dosen | |
| dc.contributor.author | DWI RAHMAT NOARI | |
| dc.date.accessioned | 2024-05-22T07:38:39Z | |
| dc.date.available | 2024-05-22T07:38:39Z | |
| dc.date.issued | 2012-08-09 | |
| dc.description.abstract | Penyakit disentri dapat disebabkan oleh Shigella dysentriae. Rerumputan dari famili Poaceae, Cyperaceae, dan Rubiaceae yang umum digunakan sebagai pakan ternak diketahui memiliki aktivitas antidibakteri. Oleh karena itu tujuan penelitian ini adalah untuk mengetahui golongan kimia simplisia dan ekstrak, mengetahui aktivitas, konsentrasi hambat tumbuh minimum (KHTM), dan konsentrasi bunuh minimum (KBM) rerumpututan pakan ternak tersebut terhadap S. dysentriae galur murni dan empat isolat multiresisten. Pengujian aktivitas dilakukan dengan metode difusi agar, penentuan nilai KHTM dengan metode mikrodilusi, sedangkan penentuan KBM dilakukan dengan subkultur ke media agar dari hasil mikrodilusi. Hasil penelitian menunjukkan bahwa secara umum rerumputan uji mengandung golongan kimia flavonoid, polifenol, steroid, dan kuinon, serta seluruh ekstrak rerumputan uji memiliki aktivitas terhadap seluruh bakteri uji dengan kisaran konsentrasi KHTM – KBM pada 0,3125 – 10% (b/v). Dysentery could be caused by Shigella dysentriae. Grass of the family Poaceae, Cyperaceae, and Rubiaceae which were commonly used as cattle feed reported containingantibacterial activity. Therefore, the purpose of this study was to determine the chemical groups of simplicia and extracts, the antibacterial activities, theminimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) of the extracts against S. dysentriae pure strains and four multiresistant isolates. The activity test was conducted using agar diffusion method, meanwhile MIC determination using microdilution method, and MBC determination by subculturing microdilution result onto agar media surface. The results showed that in general the test grasses contained chemical classes of flavonoids, polyphenols, steroids, and quinones, and the whole test grass extract had activity against all test bacteria with MIC – MBC range at 0.3125 to 10% (w/v).Key words: Shigella dysentriae, grass. | |
| dc.identifier.uri | https://repository.unpad.ac.id/handle/kandaga/260110080019 | |
| dc.subject | Shigella dysentriae | |
| dc.subject | rumput | |
| dc.subject | Tidak ada keyword | |
| dc.title | PENGUJIAN AKTIVITAS ANTIBAKTERI RERUMPUTAN PAKAN TERNAK TERHADAP Shigella dysentriae |
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