EFEK AAPTAMINE TERHADAP EKSPRESI MRNA PPARG PADA DIFERENSIASI ADIPOSIT MENGGUNAKAN LINI SEL 3T3-L1

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dc.contributor.advisorEko Fuji Ariyanto
dc.contributor.advisorPutri Halleyana Adrikni Rahman
dc.contributor.authorIBNU WIJAYA
dc.date.accessioned2024-06-11T05:35:59Z
dc.date.available2024-06-11T05:35:59Z
dc.date.issued2023-07-14
dc.description.abstractBackground and Objective: Regarding World Health Organization (WHO) data, the number of obesity cases had tripled since 1975. The diversity of marine environments, including aaptamine, has provided excellent prospects for human living for a few years. Aaptamine was considered to have an adipogenic effect. This study intends to investigate the inhibitory effect of aaptamine in inhibiting mRNA expression of Pparg on adipocyte differentiation using 3T3-L1 cell line. Materials and Methods: MTT Assay was performed to determine the non-toxic concentration of aaptamine. Three sets of 3T3-L1 preadipocytes were prepared with varying concentrations of aaptamine (0 µM, 25 µM, and 50 µM). On day 0, each well received a cocktail for adipogenic differentiation containing isobutylmethylxanthine (IBMX), dexamethasone, and insulin. Replacement of the medium proceeded every two days. On day 13, the wells were harvested for preparing qRT-PCR analysis. RNA was isolated using Quick-RNATM Miniprep Kit and cDNA was synthesized from two microliters of total RNA using SensiFASTTM cDNA Synthesis Kit. Afterward, qRT-PCR was performed using the SensiFASTTM SYBR® No-ROX Kit based on the manufacturer’s instructions. Results: The inhibition of Pparg mRNA expression by aaptamine at 25 M and 50 M (non-toxic range) concentrations in 3T3-L1 preadipocytes differed significantly (p>0.05). Conclusion: Aaptamine inhibited the mRNA expression of Pparg during the differentiation of 3T3-L1 cells.
dc.identifier.urihttps://repository.unpad.ac.id/handle/kandaga/130110200008
dc.subjectAaptamine
dc.subjectLini Sel 3T3-L1
dc.subjectEkspresi mRNA Pparg
dc.titleEFEK AAPTAMINE TERHADAP EKSPRESI MRNA PPARG PADA DIFERENSIASI ADIPOSIT MENGGUNAKAN LINI SEL 3T3-L1

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