Fakultas Kedokteran

Permanent URI for this community

https://repository.unpad.ac.id/handle/kandaga/7

Browse

Browsing Fakultas Kedokteran by Subject "Aaptamine"

Now showing 1 - 6 of 6
  • No Thumbnail Available
    Item
    AAPTAMINE EFFECT OF CANCER CELL GROWTH INHIBITION ON 3-DIMENSIONAL MODEL BREAST CANCER CELLS
    (2023-07-14) ILHAM IZDIHAR AINAN ZAHRA; Muhammad Hasan Bashari; Savira Ekawardhani
    Breast cancer remains as global health challange, representing the most commonly diagnosed cancer in woman worldwide. Breast cancer ranks first as the highest incidence of cancer surpassing lung cancer in Indonesia. HER2+ as one of the subtype of breast cancer accounts for poor prognosis in patients as well as developing resistance to first line treatment known such as trastuzumab. Aaptamine is secondary metabolite compound derived from Aaptos suberitoides has been discovered to have anti-cancer activity. This study aimed to investigate the cancer cell growth inhibition effect on 3D breast cancer cell culture (spheroid assay). HCC1954 breast cancer cell line were used as spheroids representing trastuzumab-resistant HER2+ breast cancer cell. Spheroids were grouped into negative control and serial treatment group consisting 40 µM, 80 µM, and 120 µM concentration of aaptamine. Cancer cell growth inhibition was analyzed using spheroid growth assay observed under inverted microscope with 100x magnification on day 0, 4th and 8th. This study revealed that there was statistically significant size reduction of spheroids treated with 80 µM and 120 µM compared to control group on 8th day (p value < 0.05). In conclusion, aaptamine inhibited cell growth on 3D cell culture of trastuzumab-resistant HER2+ breast cancer cells.
  • No Thumbnail Available
    Item
    Anti-Migration Effect of Aaptamine on HCC-1954 HER2 Positive Breast Cancer Cells
    (2023-07-14) KANIA ADELIA HESTY KIRANI; Resti Gradia Dwiwina; Muhammad Hasan Bashari
    Objective : This study aims to analyze aaptamine’s antimigration effect against HCC-1954 cell line, a trastuzumab-resistant HER2 positive breast cancer cells, through wound healing assay. Methods : HCC-1954 HER2 positive breast cancer cells. Aaptamine (8,9-Dimethoxy-1H-benzo[de][1,6]naphtyridine). MTT Assay were done to assess cytotoxicity of aaptamine. Wound healing assay is used to determine antimigration effect. Results : This study shows aaptamine’s capability of antimigration effect. Gap closure is compared between control group and IC5, IC10, and IC25 at 72 hours. Compared to control, results show migration area is smaller at IC25, but bigger at IC5 and IC10 though not significantly. Conclusion : Aaptamine inhibits HCC-1954 migration at IC25 though not statistically significant from control group.
  • No Thumbnail Available
    Item
    EFEK AAPTAMINE TERHADAP EKSPRESI MRNA PPARG PADA DIFERENSIASI ADIPOSIT MENGGUNAKAN LINI SEL 3T3-L1
    (2023-07-14) IBNU WIJAYA; Eko Fuji Ariyanto; Putri Halleyana Adrikni Rahman
    Background and Objective: Regarding World Health Organization (WHO) data, the number of obesity cases had tripled since 1975. The diversity of marine environments, including aaptamine, has provided excellent prospects for human living for a few years. Aaptamine was considered to have an adipogenic effect. This study intends to investigate the inhibitory effect of aaptamine in inhibiting mRNA expression of Pparg on adipocyte differentiation using 3T3-L1 cell line. Materials and Methods: MTT Assay was performed to determine the non-toxic concentration of aaptamine. Three sets of 3T3-L1 preadipocytes were prepared with varying concentrations of aaptamine (0 µM, 25 µM, and 50 µM). On day 0, each well received a cocktail for adipogenic differentiation containing isobutylmethylxanthine (IBMX), dexamethasone, and insulin. Replacement of the medium proceeded every two days. On day 13, the wells were harvested for preparing qRT-PCR analysis. RNA was isolated using Quick-RNATM Miniprep Kit and cDNA was synthesized from two microliters of total RNA using SensiFASTTM cDNA Synthesis Kit. Afterward, qRT-PCR was performed using the SensiFASTTM SYBR® No-ROX Kit based on the manufacturer’s instructions. Results: The inhibition of Pparg mRNA expression by aaptamine at 25 M and 50 M (non-toxic range) concentrations in 3T3-L1 preadipocytes differed significantly (p>0.05). Conclusion: Aaptamine inhibited the mRNA expression of Pparg during the differentiation of 3T3-L1 cells.
  • No Thumbnail Available
    Item
    Efek Penghambatan Proliferasi Aaptamin melalui Perubahan Siklus Sel Kanker Payudara Tipe HER2 Positif HCC-1954
    (2023-07-14) NIKITA LUTHFI ADRIYANA; Muhammad Hasan Bashari; Eko Fuji Ariyanto
    HER2 positive breast cancer has a high growth and proliferation rate because of HER2 protein activity. Therefore, this type of cancer has a worse clinical manifestation also prognosis. Especially now that trastuzumab-resistant cells are starting to develop, for instance, HCC-1954 cells. The aaptamine compound has the potential as an anticancer agent because it has an antiproliferative effect. Aaptamine inhibited the growth of chronic myeloid leukemia (CML) K562 cells, cytotoxic to cervical cancer HeLa cells and murine lymphoma L5178Y. This study aimed to investigate the impact of aaptamine on inhibiting proliferation through changes in the cell cycle of breast cancer HER2 positive HCC-1954. Cell cycle distribution of HCC-1954 cells was analyzed using flow cytometry. Aaptamine concentrations used in this study were IC50 and IC75 values. Our analysis also conducted a morphological observation to distinguish the density of cell control. The data obtained were interpreted using the Watson model on FlowJo software. The data from this study suggest that the aaptamine compound caused the cell accumulation at the S phase and G2/M interface arrest of HER2+ BC HCC-1954 cells significantly at a concentration of 100 μM.
  • No Thumbnail Available
    Item
    EFFECTS OF AAPTAMINE ON LIPID ACCUMULATION DURING ADIPOCYTE DIFFERENTIATION USING 3T3-L1 CELL LINE
    (2023-07-22) AGUNG PUTRI MAYASARI BHASKARA; Eko Fuji Ariyanto; Putri Halleyana Adrikni Rahman
    Background and Objective : Obesity and overweight have been a major health problem contributing to various range of noncommunicable disease and its complication. Aaptamine contained in marine sponge considered to have potential effects on lipid accumulation. The goal of this study is to determine wheter Aaptamine can inhibit lipid accumulation during adipocyte differentiation using 3T3-L1 cell line. Materials and Methods : This study used 3T3-L1 cell line in three different groups of treatment (0 μM, 25 μM, and 50 μM of Aaptamine). The cells then cultured to grow for 48 hours in DMEM medium with 10% FBS until 100% confluent. Then the cells were induced with MDI cocktail (0.5 mM methylisobutylxanthine, 1 μM dexamethasone, and 10 μg/mL insulin). The next two days, insulin was added to the medium. The medium then replaced every two days continuously until day 12. On day 13, the mature adipocyte cells were able to be stained with Oil Red O and observed under the microscope with 40x and 100x magnification. The absorbance value which determined the lipid accumulation was also measured using spectrophotometre at 550 nm wavelength. Results : Macroscopically, decreasing red stained of lipid accumulation was noticeable in treatment group at 25 μM and 50 μM respectively, compared with the control group (0 μM). Microscopically, under the 40x and 100x magnification, the control group showed the most lipid accumulation, followed with lesser lipid accumulation at 25 μM and 50 μM . The absorbance value of lipid accumulation in each treatment showed the highest value at control group (0 μM), followed with decreasing value at 25 μM and 50 μM respectively. Statistically, very significant difference between control (0 μM, 25 μM and 50 μM Aaptamine) was found with p value <0.01. Conclusion : The addition of Aaptamine into 3T3-L1 cell line during adipocyte differentiation very significantly decrease lipid accumulation. Keywords : 3T3-L1 Cell Line, Adipocyte Differentiation, Aaptamine, Lipid Accumulation
  • No Thumbnail Available
    Item
    PENGARUH AAPTAMINE TERHADAP KEMATIAN SEL KANKER PAYUDARA HER2 POSITIF HCC1954
    (2023-07-14) TETRI TUHFAHANIFA; Resti Gradia Dwiwina; Muhammad Hasan Bashari
    Latar belakang: Kanker payudara memiliki insidensi tertinggi di dunia diantara jenis kanker lainnya melebihi kanker paru. Salah satu subtipe kanker payudara, HER2 positif, diketahui memiliki prognosis yang buruk dan terjadi resistensi trastuzumab pada beberapa kasus. Aaptamine diketahui memiliki potensi sebagai agen anti kanker. Tujuan dari penelitian ini adalah untuk menyelidiki efek kematian sel aaptamine pada sel kanker payudara HER2 positif dengan resistensi trastuzumab HCC1954. Metode: HCC1954 diberi perlakuan dengan konsentrasi aaptamine 75 µM dan 100 µM. HCC1954 digunakan sebagai model kanker payudara positif HER2 resisten trastuzumab. Pengaruh aaptamine terhadap kematian sel dianalisis dengan flow cytometry menggunakan Annexin V dan Pi. Hasil: Data menunjukkan bahwa sel yang diberi aaptamine 100 µM memiliki persentase Annexin V+ yang lebih tinggi secara signifikan (25,03%) dibandingkan dengan kelompok kontrol (4,69%) dengan nilai p 0,05. Kesimpulan: Studi kami menyimpulkan bahwa aaptamine tidak berpengaruh terhadap kematian sel pada sel kanker payudara positif HCC1954 HER2 dengan resisten trastuzumab berdasarkan analisis flow cytometry.