EFFECTS OF AAPTAMINE ON LIPID ACCUMULATION DURING ADIPOCYTE DIFFERENTIATION USING 3T3-L1 CELL LINE
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2023-07-22
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Abstract
Background and Objective : Obesity and overweight have been a major health problem contributing to various range of noncommunicable disease and its complication. Aaptamine contained in marine sponge considered to have potential effects on lipid accumulation. The goal of this study is to determine wheter Aaptamine can inhibit lipid accumulation during adipocyte differentiation using 3T3-L1 cell line. Materials and Methods : This study used 3T3-L1 cell line in three different groups of treatment (0 μM, 25 μM, and 50 μM of Aaptamine). The cells then cultured to grow for 48 hours in DMEM medium with 10% FBS until 100% confluent. Then the cells were induced with MDI cocktail (0.5 mM methylisobutylxanthine, 1 μM dexamethasone, and 10 μg/mL insulin). The next two days, insulin was added to the medium. The medium then replaced every two days continuously until day 12. On day 13, the mature adipocyte cells were able to be stained with Oil Red O and observed under the microscope with 40x and 100x magnification. The absorbance value which determined the lipid accumulation was also measured using spectrophotometre at 550 nm wavelength. Results : Macroscopically, decreasing red stained of lipid accumulation was noticeable in treatment group at 25 μM and 50 μM respectively, compared with the control group (0 μM). Microscopically, under the 40x and 100x magnification, the control group showed the most lipid accumulation, followed with lesser lipid accumulation at 25 μM and 50 μM . The absorbance value of lipid accumulation in each treatment showed the highest value at control group (0 μM), followed with decreasing value at 25 μM and 50 μM respectively. Statistically, very significant difference between control (0 μM, 25 μM and 50 μM Aaptamine) was found with p value <0.01. Conclusion : The addition of Aaptamine into 3T3-L1 cell line during adipocyte differentiation very significantly decrease lipid accumulation.
Keywords : 3T3-L1 Cell Line, Adipocyte Differentiation, Aaptamine, Lipid Accumulation
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3T3-L1 Cell Line, Adipocyte Differentiation, Aaptamine