Efek Penghambatan Proliferasi Aaptamin melalui Perubahan Siklus Sel Kanker Payudara Tipe HER2 Positif HCC-1954
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Date
2023-07-14
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Abstract
HER2 positive breast cancer has a high growth and proliferation rate because of HER2 protein activity. Therefore, this type of cancer has a worse clinical manifestation also prognosis. Especially now that trastuzumab-resistant cells are starting to develop, for instance, HCC-1954 cells. The aaptamine compound has the potential as an anticancer agent because it has an antiproliferative effect. Aaptamine inhibited the growth of chronic myeloid leukemia (CML) K562 cells, cytotoxic to cervical cancer HeLa cells and murine lymphoma L5178Y. This study aimed to investigate the impact of aaptamine on inhibiting proliferation through changes in the cell cycle of breast cancer HER2 positive HCC-1954. Cell cycle distribution of HCC-1954 cells was analyzed using flow cytometry. Aaptamine concentrations used in this study were IC50 and IC75 values. Our analysis also conducted a morphological observation to distinguish the density of cell control. The data obtained were interpreted using the Watson model on FlowJo software. The data from this study suggest that the aaptamine compound caused the cell accumulation at the S phase and G2/M interface arrest of HER2+ BC HCC-1954 cells significantly at a concentration of 100 μM.
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Aaptamine, Antiproliferation, HER2 positive breast cancer