Respon Bibit Kakao (Theobroma cacao L.) Asal Somatic Embryogenesis Terhadap Komposisi Media Tanam Yang Berbeda

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2011

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1 RESPONS BIBIT KAKAO (Theobroma cacao L.) ASAL SOMATIC EMBRYOGENESIS TERHADAP KOMPOSISI MEDIA TANAM YANG BERBEDA Wulan Kumala Sari Program Magister Ilmu Tanaman, Fakultas Pertanian Universitas Padjadjaran e-mail : wulan_kumala@yahoo.com ABSTRACT One of the critical success cocoa cultivation was used of quality plant materials. Cocoa plant material obtained through propagation techniques Somatic Embryogenesis (SE) was the latest method that can produce planlet that are the same with parents, have a taproot and a branched structure similar with plant from seeds. Another advantage of this SE technique can be massproduced seedlings, pest-disease free, growth is more uniform, more vigor, high yield and drought resistant. The objective of the experiment was to know the growth media composition which gave the best effect on growth cacao seedling (Theobroma cacao L.) from Somatic Embryogenesis (SE). The experiment was conducted at The Agricultural Experiment Station of Agriculture Faculty, Padjadjaran University, Jatinangor, altitude ±700 m above sea level with soil type is Inceptisols. The experiment was conducted from June until October 2013, used Randomized Block Design (RBD), consisted of nine treatments combinations and replicated three times. The treatments were topsoil : casting (2 : 1) + urea 0,5 g application-1, topsoil : cattle dung (2 : 1) + urea 0,5 g application-1, topsoil : cacao pods compost (2 : 1) + urea 0,5 g application-1, topsoil : casting (2 : 1) + urea 0,75 g application-1, topsoil : cattle dung (2 : 1) + urea 0,75 g application-1, topsoil : cacao pods compost (2 : 1) + urea 0,75 g application-1, topsoil : casting (2 : 1) + urea 1 g application-1, topsoil : cattle dung (2 : 1) + urea 1 g application-1, topsoil : cacao pods compost (2 : 1) + urea 1 g application-1. The result of the experiment showed that the growth media composition consist of topsoil : casting (2 : 1) + urea 0,75 g application-1 gave better effect on vegetative growth of cacao seedling from SE compared to other treatment combinations, increased stem diameter, leaves number, shoot dry weight and total plant dry weight at 16 WAP is 17,54 g. Keywords : cacao seedling, Somatic Embryogenesis, growth media composition, nitrogen

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