THE ANTIFUNGAL EFFECT OF Nigella sativa (BLACK SEED) OIL AGAINST Candida albicans IN VITRO
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Date
2012-07-23
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Abstract
Nigella
sativa is often used as a natural remedy for some
diseases. Nigella sativa is known to
have many active substances. The volatile oil of Nigella sativa contains the substances: thymoquinone,
dithymoquinone, thymohydroquinone, thymol, and tannin which are considered to
inhibit the growth of bacteria, fungi, and parasites.
Infectious disease is
still one of a major health problems. Candidiasis is a mycotic infection caused
by members of the genus Candida. About 70% of all human Candida infections are caused by Candida albicans. Candidiasis are include
superficial (cutaneous or mucosal) infection and systemic infection.
This research is aimed
to know the antifungal effect of Nigella
sativa oil towards Candida albicans.
The study was done by descriptive
laboratory study. The experiment is done to determine the inhibition zone of Nigella
sativa against Candida albicans using Modified Kirby Bauer
Susceptibility Test method. Continue with determination of the Minimum
Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC).
The result shows inhibition zone on the Sabouraud agar of Candida
albicans. The mean diameter of inhibition zones are 11.0 mm, 11.7 mm, 12.0 mm,
13.0 mm, 13.7 mm, 14.8 mm, and 15.3 mm for Nigella sativa concentration
of 40%, 50%, 60%, 70%,80%, 90% and 100%, respectively. In the MIC and MFC
tests, the procedure cannot be done and the
results cannot be obtained because the fungal suspension cannot be mixed
homogenously with the Nigella sativa
oil.
The result shows that Nigella sativa oil has an antifungal
effect on Candida albicans.
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Keywords
Nigella sativa, Candida albicans, Antifungal Effect